Hello, I am a student and I need to use this GC for some basic experiments. Although i have understood how it works, and the principle behind the technique, I am not able to get good answer that how can I get yield and conversion from the peak area and retention time in GC? I have access to a SRI 8610C GC with TCD and FID detectors. After reading some posts here in the forum I learned that I need to first get the standard peaks, from which I surmised that I need to inject pure sample of each and every component that I am expecting to see in the mixture of my test sample, right? And once I save them, and then inject my test sample and then what? Could anyone please guide me through the steps to be followed?

In case, there a post and I missed it, kindly direct me there. I will really appreciate your help. Rtrt228 wrote: Hello, I am a student and I need to use this GC for some basic experiments. Although i have understood how it works, and the principle behind the technique, I am not able to get good answer that how can I get yield and conversion from the peak area and retention time in GC? I have access to a SRI 8610C GC with TCD and FID detectors.

GC and AT content of 5′ UTRs, 3′ UTRs and coding exons of RefSeq gene models biology Davo February 9, 2012 0 Firstly download bed tracks of the 5′ UTR, 3′ UTR and coding exons from the UCSC Table Browser. Binning sequence reads by GC content. 1 Perl program printing full.fasta file sequences to file, but trying to achieve specific nucleotide count with. Testing bioinformatics perl scripts. Let's write a perl module GC.pm that contains a subroutine to calculate the GC content of a DNA.

After reading some posts here in the forum I learned that I need to first get the standard peaks, from which I surmised that I need to inject pure sample of each and every component that I am expecting to see in the mixture of my test sample, right? How i met your mother episode list. And once I save them, and then inject my test sample and then what?

Could anyone please guide me through the steps to be followed? In case, there a post and I missed it, kindly direct me there. Windows server 2008 r2 standard.

I will really appreciate your help. Welcome to the forum. I don't want to sound churlish, what with it being your first post, but these really are the sorts of questions that, as a student, your supervisor / lecturer / practical demonstrator should be able to answer in a lot less time that it will take people on the forum to type it all out. They get paid to teach you - we are all doing it for nothing. If you are genuinely stuck, and have asked the questions and got no answers than come back for another try. Yield and conversion are the easy part of your question. Once you know the concentration of each compund in the mixtures, it is an easy caclulation.

Hi Don, First of all, thank you very much for the much needed detailed reply. I am now grateful to get your help. Out of the things you have listed I dont know (or have less knowledge) about the following things: -be able to develop a separation of the various components in the mixture -select and internal or external calibration method (and know why you selected one or the other) > I have what they are theoretically. Internal cali. Method you add known substance at a constant concentration to all standards and samples. And that is always present at a const amt.